| 1. | 1 vertor , dh5a competent cells and long template expanded pcr kit were from invitrogen company
1载体， dh5a感受态细菌及pcr扩增试剂购自invitrogen公司。 |
| 2. | Ligate the insert hbrp to a pgex - 5x - 1 expression vector . prepare eoli . bl21 competent cells
制备eoli . bl21感受态细胞，将pgex一hb即表达质粒，转化bl一21感受态细胞。 |
| 3. | Mineral oils stay at the injection site and are progressively eliminated by competent cells like macrophages
矿物油留在注射处象巨噬细胞这样有反应能力的细胞使得排泄量日益增多。 |
| 4. | Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
Pgem一t载体，感受态细菌jm109 ，反转录试剂盒和体外翻译试剂盒均购自promega公司。 |
| 5. | Effects of blocking in vivo cd80 cd86 costimulatory signal at the early gestation on the costimulatory molecules expression of immuno - competent cells in the abortion - prone model
86协同刺激信号对自然流产模型免疫活性细胞协同刺激分子表达的影响 |
| 6. | The ligation products were transfected to jm109 competent cells . the positive clones were confirmed by pcr , then the positive plasmids were minipreped , digested and sequenced
线性化质粒，分别用竹和sp6rna多聚酶，掺人3 ， s一dutp ，体外转录正义和反义探针。 |
| 7. | Then the linked products were transformed into the high competent cell of e . coli dh5a . based on - complementation of the detective - galactosidase , positive recombinant clone were screened from x - gal plate
从引物和基因序列的比对分析结果看， zhyf006序列与上下游引物的配对比例分别为s4 |
| 8. | Cloning and analyzing of the full - length cdna sequence the fragments consistent with expected length from the product of race - pcr reaction are separated , purified and recovered , after which the fragments are cloned into the vector of pmd - 1st to transform the competent cells
山na的克隆和测序将得到的3 ’一及5 ’ scepcr产物符合预期长度的片段，分离后纯化、回收，克隆到ta克隆载体pmd 18t ，转化e |
| 9. | The suitable concentration of plasmid should be used in transformation , when the size of plasmids was getting larger the transformation efficiency was getting lower . the competent cells with high efficiency could be used with large plasmids and the normal competent cells used with small plasmids
结果表明：对转化效率影响的主要因素是质粒浓度和质粒大小；质粒浓度越低，转化效率越低，当浓度达到饱和后，继续增加质粒浓度，转化效率下降。 |
| 10. | By blasting the homologous sequences in genbank databases , the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp . the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid . the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid , the pgcgh was transformed into e . coli bl21 competent cells
将得到的序列在genbank和embl数据库中进行了同源比较，结果显示：本研究克隆到的草鱼gh基因与genbank中登记的x60474草鱼gh基因有12个碱基的差异，编码的氨基酸有3个氨基酸残基的差异，同源性为98 ，影响蛋白质高级结构的保守二硫键为2个。 |
